Tuesday, November 26, 2019

Suicide and Musical Influence essays

Suicide and Musical Influence essays In his article, "I'd sell you suicide': pop music and moral panic in the age of Marilyn Manson," Robert Wright looks to the problems with suicide from past and current hard rock music. There are many concerns that have come up with the current wave of popular music. Suicide and depression are some of the major themes in in most song lyrics throughout hard rock and heavy metal music. Suicide became an issue in the 1960's when serious problems, such as war, race relations, feminism and ecology were present (Wright 367). Neoconservatives felt that the lyrics in the music being played was prompting "sexism, profanity, satanic influences and drug glorification" (Wright 370). Ozzy Osbourne was the first heavy metal artist to be charged in court for the lyrical content of his music. The charges were dropped based on the first amendment which protect his right to freedom of speech in his song lyrics. Other cases had been linked to suicide with subliminal messages that teenagers heard in t he musics lyrics as well. Those who filed the suits stated that subliminal messages are what led their children to commit suicide. The 1980s had the highest number of teenage suicides ever recorded, and today, suicide continues to be one of the leading causes of teenage deaths. None of the suicidal cases have ever been linked with rock musics lyrical content. Using the art of rhetoric, Wright believes that the relationship of popular culture and public morality shows that rock music is not the cause of teen suicide, rather society in general is the root foundation of this problem. Wright, born in the late 1960s spent his early childhood in the middle of the beginning of the socioeconomic malaise. Popular culture began to arise, and produced heightened individualism, nonconformity and escapism, rather than the reverse (Wright 366). This time period did not articulate publicly the issues of poverty, drug abuse, and inne...

Friday, November 22, 2019

Photosynthesis Study Guide - Key Concepts

Photosynthesis Study Guide - Key Concepts Learn about photosynthesis step-by-step with this quick study guide. Start with the basics: Quick Review of the Key Concepts of Photosynthesis In plants, photosynthesis is used to convert light energy from sunlight into chemical energy (glucose). Carbon dioxide, water, and light are used to make glucose and oxygen.Photosynthesis is not a single chemical reaction, but rather a set of chemical reactions. The overall reaction is:6CO2 6H2O light → C6H12O6 6O2The reactions of photosynthesis can be categorized as light-dependent reactions and dark reactions.Chlorophyll is a key molecule for photosynthesis, though other cartenoid pigments also participate. There are four (4) types of chlorophyll: a, b, c, and d. Although we normally think of plants as having chlorophyll and performing photosynthesis, many microorganisms use this molecule, including some prokaryotic cells. In plants, chlorophyll is found in a special structure, which is called a chloroplast.The reactions for photosynthesis take place in different areas of the chloroplast. The chloroplast has three membranes (inner, outer, thylakoid) and is divided into th ree compartments (stroma, thylakoid space, inter-membrane space). Dark reactions occur in the stroma. Light reactions occur the thylakoid membranes. There is more than one form of photosynthesis. In addition, other organisms convert energy into food using non-photosynthetic reactions (e.g. lithotroph and methanogen bacteria)Products of Photosynthesis Steps of Photosynthesis Here is a summary of the steps  used by plants and other organisms to use solar energy to make chemical energy: In plants, photosynthesis usually occurs in the leaves. This is where plants can get the raw materials for photosynthesis all in one convenient location. Carbon dioxide and oxygen enter/exit the leaves through pores called stomata. Water is delivered to the leaves from the roots through a vascular system. The chlorophyll  in the chloroplasts inside leaf cells  absorbs sunlight.The process of photosynthesis  is divided into two main parts: light dependent reactions and light independent or dark reactions. The light dependent reaction happens when  solar energy  is captured to make a molecule called ATP (adenosine triphosphate). The dark reaction happens when the ATP is used to make glucose (the Calvin Cycle).Chlorophyll and other carotenoids form what are called antenna complexes. Antenna complexes transfer light energy to one of two types of photochemical reaction centers: P700, which is part of Photosystem I, or P680, which is part of Photosystem II. The photochemical reac tion centers are located on the thylakoid membrane of the chloroplast. Excited electrons are transferred to electron acceptors, leaving the reaction center in an oxidized state. The light-independent reactions produce carbohydrates by using ATP and NADPH that was formed from the light-dependent reactions. Photosynthesis Light Reactions Not all wavelengths of light are absorbed during photosynthesis. Green, the color of most plants, is actually the color that is reflected. The light that is absorbed splits water into hydrogen and oxygen: H2O light energy →  ½ O2   2H   2 electrons Excited electrons from Photosystem I can use an electron transport chain to reduce oxidized P700. This sets up a proton gradient, which can generate ATP. The end result of this looping electron flow, called cyclic phosphorylation, is the generation of ATP and P700.Excited electrons from Photosystem I could flow down a different electron transport chain to produce NADPH, which is used to synthesize carbohydratyes. This is a noncyclic pathway in which P700  is reduced by an exicted electron from Photosystem II.An excited electron from Photosystem II flows down an electron transport chain from excited P680  to the oxidized form of P700, creating a proton gradient between the stroma and thylakoids that generates ATP. The net result of this reaction is called noncyclic photophosphorylation.Water contributes the electron that is needed to regenerate the reduced P680. The reduction of each molecule of NADP  to NADPH uses  two electrons  and requires  four photons.  Two molecul es  of ATP are formed. Photosynthesis Dark Reactions Dark reactions dont require light, but they arent inhibited by it, either. For most plants, the dark reactions take place during daytime. The dark reaction occurs in the stroma of the chloroplast. This reaction is called carbon fixation or the  Calvin cycle. In this reaction, carbon dioxide is converted to sugar using ATP and NADPH. Carbon dioxide is combined with a 5-carbon sugar to form a 6-carbon sugar. The 6-carbon sugar is broken into two  sugar molecules, glucose and fructose, which can be used to make sucrose. The reaction requires 72 photons of light. The efficiency of photosynthesis is limited by environmental factors, including light, water, and carbon dioxide. In hot or dry weather, plants may close their stomata to conserve water. When the stomata are closed, the plants may start photorespiration. Plants called C4  plants maintain high levels of carbon dioxide inside cells that make glucose, to help avoid photorespiration. C4  plants produce carbohydrates more efficiently than normal C3  plants, provided the carbon dioxide is limiting and sufficient light is available to support the reaction. In moderate temperatures, too much of an energy burden is placed on the plants to make the C4  strategy worthwhile (named 3 and 4 because of the number of carbons in the intermediate reaction). C4  plants thrive in hot, dry climates.Study Questions Here are some questions you can ask yourself, to help you determine if you really understand the basics of how photosynthesis works. Define photosynthesis.What materials are required for photosynthesis? What is produced?Write the  overall reaction  for photosynthesis.Describe what happens during the cyclic phosphorylation of photosystem I. How does the transfer of electrons lead to the synthesis of ATP?Describe the reactions of carbon fixation or the  Calvin cycle. What enzyme catalyzes the reaction? What are the products of the reaction? Do you feel ready to test yourself? Take the  photosynthesis quiz!

Thursday, November 21, 2019

History of Korean Arts Essay Example | Topics and Well Written Essays - 1000 words

History of Korean Arts - Essay Example The methodology followed to have the rhetorical analysis done was to critically examine the text and the layout of the pages and to arrive at a conclusion by comparing them with the standardized theories and principles. It is quite evident from the layout and the site tree that the information is intended to be extended to a wide range of audience. It is interesting to note that there the website has made available eleven multilingual versions excluding the one in English. This clearly indicates that the objective is to expose Korean culture through its art forms and handicrafts to the people across the globe. The knowledge that the website offers can be useful to researchers, historians and also to students. The approach of the website to cater for people ranging from common man to specific researchers. In each and every detail of the WebPages the integrated purpose of the web resource is evidently visible. Be that the text or even the smallest of graphical elements, all has worked together towards the purpose of ideally reflecting Korean culture. The home page promises the reader to be taken through a breathtaking journey into the history of Korean culture. In the simplest language, the purpose of the website is to highlight Korean culture through the traditional and historical aspects pertaining to its arts and handicrafts. The arrangement and the layout also contains within it specific Korean cultural reflections. However, the arrangement of the homepage appears to be a bit complex and improper.

Tuesday, November 19, 2019

Research Proposal Paper Example | Topics and Well Written Essays - 1750 words

Paper - Research Proposal Example The understudies will be asked to finish an overview (Drummond,2014). The finished study will create the greater part of the essential parts to position every understudy in the right area of the 3 X 3 factorial outlines. The study will contain an assortment of inquiries identified with the theory of this study to focus the examples. They are between the utilization designs/ recurrence of the utilization of video games with the kind of video diversion to the understudies achievement and psychological state. The overview will likewise educate with respect to the additional curricular exercises of the understudies since this kind of out of school association might likewise relate to higher or lower government sanctioned test scores. This study is critical due to how essential state-administered testing has ended up, and the amount time and cash get put into enhancing the scores of understudies here. Technology is an apparatus that educators can use to help adapting, yet brain research tutoring must be first. In the event that instructors and therapists do not see how to help learning, engineering utilization will be ineffectual and wasteful. The key determinant of our prosperity and enthusiastic dependability will not be the quantity of machines obtained or links introduced. Instead, it is how people characterize instructive dreams, plan and help staff, outline educational program, location issues of value, and react to the quickly evolving world. Innovation gets utilized as a part of different courses than for instructive profits. Engineering got additionally created for amusement and relaxation. Video games are a type of engineering incorporating visual, sound-related, and kinesthetic/material reenactment structures. Not all, yet numerous understudies are excited in the use of video games in the timetable of relaxation time and unwinding. Video games have advanced a long ways past the

Sunday, November 17, 2019

Criminal Profiling Essay Example for Free

Criminal Profiling Essay Criminal profiling involves analyzing the scene of a crime and then use the information gathered from the analysis to determine correctly the identity of a perpetrator. This method of identifying perpetrators is very useful though it does not assist an investigator in getting the name of the perpetrator it helps narrow down and thus makes it easier to identify a criminal. (Dennis, 2006) Some of the pertinent issues that criminal profiling helps in bringing out include but not limited to the sex of the perpetrator, the background of a person in terms of ethnic community, age and personality. This profile is then used to single out a criminal among several people who appear to match with some of the features identified in the profile. One may wonder just how does criminal profiling work? Normally experts examine the manner in which a particular crime is committed. This is commonly referred to as the method of operations. Usually the experts will take the identity of the victims and then examine what the suspected criminals have in common especially in terms of the likely weapon that was used to commit the particular crime in question. The other issue that the experts look at is the brutality or hostility that was used during the commission of the crime in question by the perpetrator. Existence of torture or any form of hostility is yet another trait that experts look for when using the criminal profiling method. One student in an American University during a lecture asked her professor why he believed that the criminal profiling method actually works in identifying a perpetrator in a criminal act. The professor gave a precise answer and stated that thorough research has been carried out over the years and there have been conducted some interviews even with convicts who have helped prove the workability of this method. There are two main ways that are used to come up a criminal profile. One of the methods is one that is commonly used by the Federal Bureau of Investigation known as criminal scene analysis. This method involves a number of steps which include profiling inputs. This basically involves gathering evidence in terms of anything that was found in the scene of the crime. The second step usually is to put the evidence together and try to see if there is any common aspect in the crimes committed by the suspects. Crime assessment and investigation follows and it is after that some apprehensions can be made based on the investigations. The second method is the behavioral evidence analysis. This method was developed by Brent Turvey who argued that when a crime is being investigated there are a lot of discrepancies that rise from all the gatherings that are made by the investigative team. The first step is forensic analysis which involves analyzing physical evidenced gathered which may involve taking photographs and videos of the crime scene and having interviews with some of the victims of the crime who may be alive. Victimology is the nest step and involves creating a profile for a victim which in turn helps identify the criminal responsible. The third step is examining keenly the features and characteristics of the scene of crime as this is said to give a leading clue on who the suspect could be. The last step is combining the first three steps discussed and it is this combination that helps the investigators come up with a criminal profile. According to Turvey a crime ought to be analyzed in four main steps to ensure that the information gathered by the investigative team does not bear any discrepancies. Criminal profiling has in several instances been criticized as a source that lacks credibility on the basis that it is based on speculations. However experts state that according to research that has been conducted for several years now, criminal profiling has a considerably high level of credibility. Profiling involves a number of phases. The initial phase in every criminal profiling is known as antecedent which involves attempting to figure out the plan or the fantasy that the perpetrator of a crime had prior to the commitment of the crime. Basically this phase of criminal profiling involves an attempt to unearth the motive behind the commission of a crime by the perpetrator. The second phase is known as method and manner and involves examining the target group that the perpetrator was aiming at in the crime committed and more so the manner in which or better still the method used to commit the crime in question. Brent, 2002) The third phase in criminal profiling is known as body disposal and is common in murder cases. This is where experts attempt to examine where the scene of crime is the same with the place where the body was dumped or whether the crime was committed at a particular place and then the body dumped in yet another place thus introducing the concept of multiple. (Davis, 2007) Post offense behavior is the final or the last phase in criminal profiling and it is quite tricky. Post offense behavior involves investigating behavior of criminals especially after commission of crimes. Normally some criminals try to hide their identity by pretending to be part of the investigating team. Experts must therefore use their expert knowledge to single out criminals who pretend to be assisting in the investigation process yet they are the suspects. Having examined in great depths what criminal profiling is all about, it may be of paramount importance to address the issue of whether criminal profiling has managed to shape up chance. Basically by making this analysis we are able to know whether criminal; profiling does any play any vital role in helping to identify criminals in a given crime in question. In simpler terms using criminal profiling as a method of identifying criminals what are the chances of identifying the suspect responsible in the criminal act at hand. In the beginning of the twentieth century, criminal law took a new twist with criminal profiling slowly shaping chance. Majorly what changed during the twentieth century was the inference in terms of probability. During this century actuarial methods changed. One thing to note that it is not the risk analysis and actuarial thinking that actually changed but the inferences in terms of probability. The actuarial methods got more refined so that using the criminal profiling method one can be sure that the identity of a particular perpetrator is correct. In conclusion therefore it is correct to state that over the year and more so after the twentieth century, criminal profiling is slowly gaining acceptance as reliable method of correctly identifying perpetrators of crimes. This is because it has been studied and proved that it shapes chance so that the probability of a person to be the suspect of a crime in question having been pointed out by the criminal profiling method is very high.

Thursday, November 14, 2019

Training Environment :: essays research papers

Using your Training Environment Tips for survival Your training environment is the most essential and important element of your presentation. It is the difference between a good presentation and one that will keep the participants asking for more. The five most important features of your training environment will be: 1. White Board 2. Flipchart 3. Projector 4. Space 5. Body Language A good presenter uses his training environment in the most effective and coordinative way possible. Here are a few tips that will help you use your training environment in a more effective manner, while giving presentations. White Board The white board is your medium of writing and projecting the presentation. In the modern world, white boards have revolutionarized and have added features in built such as a play, pause and stop button for you presentations and images. They can be connected to your projector to run your presentation more effectively and easily. But as much as technology may change, the basics of the white board will stay the same, and they are: †¢Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Leave margins on the corners of the board. Do not fill up the board too much especially around the corners. Keep a spacing of 5 inches from the left/right and top/bottom corners of the board. †¢Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Always use a non-permanent marker on the white board. This ensures easy reusability of the board at various levels. †¢Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  The color of the markers should be bright and solid. Do not use faint markers as they are not clearly visible. The purpose is easy visibility, not the formality of writing. †¢Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  The letters should be at least  ½ â€Å" high and should be in capital letters. Caps case is easier to read. †¢Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Use a variety of color markers for highlighting and adding variety to what is written on the board. †¢Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  When using the white board as the projection screen make sure is cleaned and has no patches of ink. If you have written over the projection on the board then ensure that you wipe it before proceeding to the next slide. †¢Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Do not stand in front of the board if there is something written on it. Flip Chart The flip chart is your medium of that extra unit of writing and displaying your points. It is often termed as the trainers â€Å"companion†. The flip chart is mostly used as a tool for writing or illustrating points that are a part of the presentation, but may or may not be a part of the power point slides. Some key tips for using the flip chart are:

Tuesday, November 12, 2019

Determining The Longetivity Of E.Coli’s Gaining Resistance Ability: A Comparison Between The Bacteriophage T-4 And Antibiotic

The surfacing of a variety of drugs for resisting antibiotic for disease-inflicting bacteria has already been a big issue and at the same a vital dilemma in treating all types of human diseases. This immediately requires another option, a substitute way of providing therapy to the human diseases. It has been found in previous researches and experiments the wonders of having bacteriophages highly considered to be â€Å"healing viruses. † John MacGregor (2003) has brought up an intriguing issue regarding bacteriophages when he wrote his research article entitled â€Å"Set A Bug To Catch A Bug†.Apart from the title, his words were â€Å"As the power of antibiotic wanes, viruses that hijack bacteria and smash them into pieces could be the answer to our prayers†¦Ã¢â‚¬ . He explained in his article the possibilities of replacing antibiotics as the solution to a lot of virus-causing diseases including virus infections. Bacteriophages were first discovered by a British c hemist named E. H. Hankin. It was considered to be a virus in 1915 by Frederick Twort, a British bateriologisy. The occurrence of that first intrigue found by Dr.Hankin paved its way for more discoveries performed by a Canadian microbiologist named Felix d’Herelle. He agreed with Twort when he also considered it to be a virus and then later he named it as a â€Å"bacteriophage†. Upon his successful experimentations, he was confident that these bacteriophages will be very helpful and at the same time when he used them with the children who were almost dying dysentery at a hospital in Paris. The test solutions were distributed to every patient hoping that it will be effective, and fortunately, these cured the children for just one night.With D’Herelle’s primary success, the use of phage therapy was further studied. From then on, the advantages it provides were widely spread globally. These page therapies are utilized in a variety of ways. It can be taken or given topically, orally, can be injected, using enemas and aerosols. Diseases that were treated by this phage therapy included urinary tract infections, typhoid and cholera. The use of phage therapy slowly faded when AMA or the American Medical Association reported contradictory results of using phages. Antibiotic age came in when penicillin was discovered by Alexander Fleming in 1982.Fleming’s discovery flourished for 20 years making the phage therapy out of sight. But still a lot of microbiologists became very attractive to what bacteriophages could provide medically, improving different aspects of health and curing more diseases. There was a time after the Communist era when phages were considered to be the â€Å"last resort† antibiotics when the decline for antibiotics took place. This was indeed an alarming situation worldwide. But this did not become hindrance in reviving the hidden attributes of bacteriophages.Bacteriophages are defined by Toronto, Funke and Ca se (2001) as viruses that host in bacteria and in bacterial cultures, they can be grown easily. This has been significant since bacteriophages are the main sources of multiplying viruses. How do bacteriophages multiply? The basic procedure in which multiplication of viruses happens is just the same as the other viruses and it is not affected by any means by which the entering and the exiting of a virus into a host cell differs. Most life cycles of a virus are difficult to understand but a bacteriophage is an exception.They are the easiest to be learned and be understood. Bacteriophages, also called â€Å"phages†, can be multiplied using two substitute processes. The first one is called the lytic cycle which leaves the host cell to a lysis or death. The second one is called the lysogenic cycle where the host cell lives. T-even bacteriophages such as T2, T4 and T6 are the phage types that are most studied. Using the bacterium Escherichia coli (E. coli) as a host, with the use o f lytic cycle, multiplication of the T-even bacteriophages can be demonstrated easily from one process to another.There are 5 stages involved starting from attachment, penetration, biosynthesis, maturation and release. During the first stage, attachment, the particles of the bactriophage and the bacteria collide. A chemical connection occurs between the attachment site from the virus and the bacterial cells’ complementary receptor site. A chemically produced interaction from the connection enables bonds that are weak to be formed from the two sites by using their fibered tail ends. During the stage of penetration, DNA is being injected by the T-even bacteriophages into the bacterium after connecting occurs.This is done when an enzyme called the phage lysozyme is released by the tail of the bacteriophage which in turn destroys the walls of the bacterial cell. In the penetration process, the phage’s sheath tail contracts and the core of the tail enters the cell wall. If the core’s tip has already reached the plasma membrane, the DNA from the head of the bacteriophage will pass through the tail core, it will eventually enter the bacterial cell. During the stage of biosynthesis, the DNA of the bacteriophage will initiate synthesis direction of the components of the virus by the host cell.Once the components of the virus are being put in place and are brought together into virions, the maturation stage occurs. When the lyses of the host cell and the new virions have already been released, it is referred to as the release stage (Toronto, Funke and Case; 2001). Bacteriophages have been found to exhibit a lot of features. They are tested to be of big use with local infections in relation to poor supply of blood like diabetic ulcers and infections of the bone. Unlike antiobiotics, these phages were keenly observed to multiply inside their host cell which enables them to penetrate more deeply to the area being infected.Another distinguishing feature of phage therapies is its ability to inflict no allergies, resulting to a fewer side effects. Phage therapies in addition are easier and are cheaper to produce than antibiotics. On the other hand, bacteriophages have their limitations concerning their fatality once they have already killed the harmful bacteria. But these issues should not lower the hopes of the society depending on the future developments of phages. In an interview (Society for Gen. Micro. , 2008), they have shared that modern scientists and researchers have already found ways of prolonging the lifespan of viruses.This new and possible idea is by combining them chemically with polymers but still this is limiting since it will likely cause poisoning of the blood and is surely a big threat to one’s life. The main objective of this project is to determine the longetivity of the bacterium Escherichia coli’s gaining resistance ability in two different viral invaders: the bacteriophage T-4 and an antibiotic . At the end of this project, results should report a comparison between these two, answering which has the longest and the most effective invading mechanism. B. METHODS AND PROCEDURES (EXPERIMENT PROTOCOL)Throughout the following procedures of this project, a strict Aseptic Technique will be used. During the whole duration of the experiment, a strict technique called the Aseptic Technique will be applied. According to a web article research, written by Hauswirth and Sherk (2007), they defined the aseptic technique as an accumulation of unique practices acquired and a set of processes undergone whose conditions are carefully controlled with an objective of minimizing pathogen contamination. In any type of clinical setting, the technique is used to maximize and stabilize pathogenic organisms’ absence.Its main goal is to simply protect a patient from infection and cease any possible spreading to other body parts. Prevention of infections are not achieved simply by sanitizing or disinfecting. In about more than 27 million surgical operations, surgical sites are the third most prone to more hospital infections prolonging, in effect, the hospital stay of the patient and at the same time, hospital bills become so expensive. The estimate was according to CDC or Center for Disease Control and Prevention (Hauswirth and Sherk, 2007).The main objective of performing this experiment is to identify the required length of time for a common bacterium called Escherichia coli (E. coli) to achieve its maximum log phase growth. When these cells have already arrived in its expression of maximum amount of bacteriophage receptors, it will eventually lead to an immediate vulnerability to infection. The bacterium E. coli must always be utilized at this stage of growth all through out the procedures to be able to achieve desirable results upon comparing of two different experiments.In beginning the procedures of the experiment, first, an overnight growth of culture will be prep ared to be able to supply the growth curve with inoculum. The growth of the culture will be done in a shaking incubator with a temperature of 37 degrees Centigrade. The culture preparation will also be dependent on what culture is available, its slope, its colony or plate, and inoculate broth of the culture. Second, a 1 ml of culture that will be prepared overnight and a 99 ml of inoculate nutrient broth (NB) will be taken and will be placed in a flask that is sterilized and flat-bottomed type.Through a process called resuspension, a sterilized tube containing a sample of 5 ml will be gently swirled, will be collected and will be marked Time Zero. The sterilized flat-bottomed flask will be placed inside the shaking incubator. Third, samples containing 5 ml each will be collected at a per hour interval. This will be done for 8 consecutive 8 hours and will be marked Time 1, Time 2, Time 3, Time 4 . . . Time 8. All samples will be stored at +4 degrees Centigrade. Fourth, the remains of the culture that has been prepared overnight will be left for one more night.At exactly 9 am the next day, a last sample of the culture remains will be collected. Fifth, from a sample of 400 nm and another sample of 450 nm, the OD of each sample will be measured. The LB or NB will be used but will be left blank if necessary. If in case, the OD will exceed 1. 0, both sample 1 and sample 2 that were used in LB and NB will be diluted and will be read for the second time. Sixth, the strict aseptic technique will be used in preparing for colony counts in each sample. The amount of workable cells (per ml) will be identified.Seventh, plotting will be done. A growth curve will be plotted and both the cell number and time will be involved in doing this. Eighth, another growth curve will be plotted. This time cell number and OD will be involved in the plotting. Ninth, the required time to reach the midway of the log phase growth will be identified. In doing this, cells within the time length , identified prior to the succeeding experiment, will be grown. Tenth and last step of these experiment procedures, the connection between the cells and the OD will be analyzed.All results acquired all through out the process of this experiment will be recorded and will be evaluated accordingly. References: Hauswirth, K. & Sherk, S. D. (2007) Aseptic Technique [Internet]. Available from < http://www. surgeryencyclopedia. com/A-Ce/Aseptic-Technique. html> [Accessed 8 May 2008] Tortora, Funke & Case (2001) Microbiology: An Introduction. 7th ed. Addison-Wesley Longman, Inc. Craigie, J. (2002) The Significance and Applications of Bacteriophage in Bacteriological and Virus Research [Internet]. Available from [Accessed 8 May 2008]

Saturday, November 9, 2019

How Wilfred Owen and John Scott present ideas about slaughter and sacrifice Essay

Write a comparison of the ways Wilfred Owen and John Scott present ideas about slaughter and sacrifice, how far do you agree with the view that Scott’s poem is more effective than Owens in communicating its message? The Send Off and The Drum both explore the truths about war outlining the horrors and effects of war through language and poetic techniques. Owen attacks the understanding between those at home who promote war as a romantic, glorious and heroic exercise and dying for one’s country as an act of nobility and with this who fight and die in war and who know the true horror of the battlefield. Scott explores the desperations caused by battle. He also makes clear that he does not consider war as a fight of honour; more as a gruesome game to see who can be more stubborn to admit what they are doing is wrong. Both poets show similar thoughts about war and how it is betrayed to a glorious act of heroicness. In the opening stanzas of the drum the poets mention the sounds and tones which follow the soldiers. In The drums case it is the ‘drums discordant sound’. This instrument is used for the historical march of the soldiers. Which in this case is confusing to the men. As they are ‘parading round, and round’. This gives a sense of never ending war. Each day the men follow the same routine until it is there turn to face the enemy in battle. Owen immediately challenges the positive connotation of the send off in the first line. Our first image is of â€Å"close darkening lanes†. This has two meanings; the first meaning is the lanes fatefully enclose the soldiers in their closeness, they cannot turn back, the second meaning is that â€Å"darkening† is emphasized as a metaphor of the soldier’s dark destiny to which their farewell is sending them and that is to be killed in war. John Scott talks about the pathetic reasons which people go to war. He explains how the young are almost excited by the surrender of the opposition. ‘To sell their liberty for charms’. This line suggests that people would rather sell there independence for money. Regarding how it would affect their country. The alliteration used in the phrase â€Å"grimly gay† in the send off alerts the readers the forced gaiety of the men and ambiguity of their supposed adventure isn’t everything that is thought to be at first. â€Å"Grimly Gay† also contradicts the concepts of grimness and gaiety, which captures a focus for the mixture of excitement and fear in the men and the country for which they sacrifice their lives for. Scott mentions the line ‘ambition’s voice commands’. This word ambition almost represents the lives of many of the soldiers fighting in the war. Either there ambition to return to there country or to fight for there country. It can also be in replacement for the general who commands his soldiers to war. A sense of death is built up in the next line. ‘To March, and fight, and fall, in foreign lands’. This shows the reality of war, you can fight and put your life on the line, but you may also lie dead in the hands of the enemy and fall in there territory. The send off portrays the sense of something sinister and shameful about the operation, in the uncertainty of the men’s future and in the prospect of the injured and weary return of â€Å"too few† of them. Owen talks about how complete strangers to him went to war. ‘They were not ours’ this explains how he talks about random people being in the war. The imagery for the gifts of flowers for good fortune is mixed with that of flowers for a funeral wreath. It is as though the gift of flowers is the soldiers first war wound, a prelude to a course of events that must end in death. Scott shows exactly what the true meaning to war is and how it affects the life of others. He speaks of the ‘burning towns’ ‘mangled limbs’ ‘windows tears’. These quotations explain the sense of horror and slaughter which took place in the Napoleonic wars. The way Scott expresses his views on the war are the tragic ways in which widows had to cope without there husband. Those who lost there homes and had to migrate to another area. Owen shows this kind of horror as well but not in the magnitude of Scott. ‘ As men’s dead’. The apostrophe in the word men shows the extensive number of soldiers who were killed. Wilfred Owen ends his poem almost like the ending of a war and how they leave the allies country. ‘Shall they return to beating of great bells, in wild train loads? A few, a few, too few for drums and yells’. The word ‘shall’ suggest that you do not really know if soldiers will return in a good way. He almost contradicts himself when he talks about loads of men returning then he says a few. This shows that not even he is sure about the outcome of returning soldiers. Part of the soldier’s betrayal is how they are dehumanized and treated so indifferently by the authorities that process them. Terms such as â€Å"siding-shed† and â€Å"train-loads† creates the concept of produce rather than people being transported. The structure of the poem is broken up into stanzas with lines long and short. The shorter lines are a lot more sinister in tone which talks about ominous events that are going to happen in war. The lengthier lines are more informative. This irregularity of long and short lines and stanzas gives the poem motion which shows the beat of marching men and a moving train. The structure of the drum is completely different it is set out in two stanzas which echo the beat of the drum which is parading around and around. That is why the use of repetition is applied for this line in both stanzas. I agree in a sense that Scott’s poem is more effective on its approach to get its message through to the reader. The reason for this being is that it can relate with the reader more because he is writing a personal response. Noticeable by the use of ‘I’ this means that he can communicate in a more individual way. He also gives more ideas on what soldiers and families would have had to see through the war stages. Not only does he give a personal statement but takes into consideration of how it may have affected the lives of others. He also shows the gruesomeness which occurs in battle. However Owen also has written a very informative poem. As he has the experience of being a solider he has more of an idea how the war worked. Unlike John Scott he did not oppose to violence. Maybe that is why Scott could have been biased towards the idea of war. Only pin pointing the parts to war which were bad, and not those which commemorate the honour and remembrance which you receive after fighting in the war. But Owen also feels the same way about the war and he has been there. I suppose these poems would not be similar because they are both from different stages of time. One which was written in the Napoleonic war and the other in world war one. That is why there is a difference in writing styles and issues which are discussed. The reason for John Scott’s poem being more violent maybe because they did not use weapons like guns and explosives so battles with swords maybe have been more brutal. Taking this into consideration I think both poems have different ways in which to get there message across so they are equally as effective.

Thursday, November 7, 2019

The U.S. Pledge of Allegiance in German

The U.S. Pledge of Allegiance in German One of the best ways to learn German is to use something that youre already familiar with. For German students in the United States, the Pledge of Allegiance is a great lesson that can be tailored to beginners and advanced students.   The majority of American students grow up citing the Pledge of Allegiance (Der amerikanische Treueschwur). Its fixed in our memories from a very young age, so learning it in German can really help students understand and practice grammar, pronunciation, and vocabulary in a single and recognizable sentence. U.S. Pledge of Allegiance(DerAmerikanischeTreueschwur) In this instance, we use der Treueschwur  for the  English word  and the U.S. Pledge of Allegiance translates to der  amerikanische  Treueschwur  or  Treueschwur der USA. Taking those famous words, I pledge allegiance... into German is a matter of finding the right vocabulary and placing it in the correct word order. The Pledge can be an excellent lesson for students of all levels. Beginners can use it to practice German pronunciation and learn some new vocabulary while reciting it with the familiar cadence. Intermediate students can use it to study word order and proper German grammar. Advanced students can make their own attempts to translate the Pledge into German own, then compare it to the examples given. Keep in mind that translation from one language to another is never perfect or word for word. As you can see in the two examples, different words can mean the same thing. For instance,  schwà ¶re  means swear and  gelobe  means vow, but theyre both used for the verb pledge. Another example is the words  jeden  (each) and  alle  (all).  They both can be used to mean everyone, which is what the Pledge implies by all. It should be noted, however, that the first translation is the more widely accepted version of the two. German translation 1: „Ich schwà ¶re Treue auf die Fahne der Vereingten Staaten von Amerika und die Republik, fà ¼r die sie steht, eine Nation unter Gott, unteilbar, mit Freiheit und Gerechtigkeit fà ¼r jeden.â€Å" German translation 2: „Ich gelobe Treue der Fahne der Vereingten Staaten von Amerika und der Republik, fà ¼r die sie steht, eine Nation unter Gott, unteilbar, mit Freiheit und Gerechtigkeit fà ¼r alle.â€Å" The Pledge of Allegiance: â€Å" I pledge allegiance to the flag of the United States of America and to the Republic for which it stands, one nation under God, indivisible, with liberty and justice for all.† Who Wrote the U.S. Pledge of Allegiance? The Pledge of Allegiance was written by Baptist minister and socialist Francis Bellamy. It first appeared in The Youths Companion  magazine in 1892 to commemorate the 400th anniversary of the discovery of America. The original oath used the phrase â€Å"my flag† rather than â€Å"the flag of the United States of America.† The change was made in 1923. The next alteration occurred in 1954 when Congress inserted the phrase â€Å"under God.† It is interesting to note that, according to his granddaughter, Bellamy himself would have objected to this religious amendment. Additionally, the author had  originally wanted to include the word â€Å"equality† in front of â€Å"liberty and justice.† He reluctantly left that word out because he felt it controversial. Equality did not seem right to him given the fact that women and African Americans were not considered equal by many people in 1892.

Tuesday, November 5, 2019

The Perils of Writing to Someone You Dont Know

The Perils of Writing to Someone You Dont Know The Perils of Writing to Someone You Dont Know The Perils of Writing to Someone You Dont Know By Maeve Maddox From a reader: I have a question.   I work for a large Canadian law firm and Ive noticed that many of the people here do not use Mr. or Ms., but rather address letters to John Smith.  Ã‚  Have I missed something?   Is this proper now? It may not be proper, but sometimes its safer. Addressing a letter in the old days was a fairly straightforward undertaking. If the name of the person was Michael Jones, youd say Dear Sir, or Dear Mr. Jones. If you were writing to a woman you could safely address her, married or unmarried, as Dear Madame. Back when the great goal of most women was to find Mr. Right, you could take a chance on starting a letter Dear Mrs. Jones even if you didnt know the recipients marital status. If she wasnt married, shed probably giggle at the error. In these more enlightened days, knowing how to begin a letter to a person you dont know is like walking through a minefield. Current letter-writing guidelines will tell you to address a man as Mr. Jones and a woman as Ms. Jones, but thats pretty simplistic, given the complications of feminism, reactions to feminism, multiculturalism, and creative child-naming. Ideally, the letter writer will check out the person being written to so as to know what form of address to use. But what if, despite your best efforts, you just dont know? How, for example, would you start a letter to Drew Barrymore, Daryl Hannah, or Michael Learned if you dont know all three are women? How are you supposed to figure out the gender of Jordan Dane, Alex Wright, Cory Black. Elliot Simpson, or Jamie Johnson if you know nothing about them but their names? In the age of the world-wide web, what about a name like Ananda Singh? A name ending in -a looks feminine to me, but in India, Ananda is a guys name. Say that you do know that the person youre writing to is a woman. Are you sure you want to address her as Ms. Jones? She may have a Ph.D. and prefer Dr. Jones. She may be a reactionary housewife and detest being called Ms. Or maybe shes single and proud of it and wants everyone to address her as Miss Jones. The people at your law firm are probably less concerned about proper usage than they are about avoiding ruffled feathers. Sometimes the best course of action is to play it safe by using the full name instead trying to juggle a courtesy title. Want to improve your English in five minutes a day? Get a subscription and start receiving our writing tips and exercises daily! Keep learning! Browse the Business Writing category, check our popular posts, or choose a related post below:70 Idioms with Heart50 Idioms About Roads and PathsComma After Introductory Phrases

Sunday, November 3, 2019

Angel response Essay Example | Topics and Well Written Essays - 250 words

Angel response - Essay Example in his character, in his whole nature, there was something attractive and elusive which allured women and disposed them in his favor; he knew that, and some force seemed to draw him, too, to them.† (Chekhov 1.5) Gurov also comes out as very judgmental where we see him refereeing to women as the lower race. Gurov’s relationship is founded by his past life and experience. Gurov is very important as the author uses him to develop almost every theme and depicts the main theme of a loveless marriage. Anna is no more of a main character as we spend less time with her as compared to Gurov. All we know about her is that she is 22 years old, married at a tender age and does not enjoy her marriage. Even the first time she has sex with her husband she was not satisfied. Anna just like her husband is eager to life â€Å" I have been tormented by curiosity† she said in one of the conversation (Chekhov 6) .Anna clearly contributes to the story in many ways which include where her unhappiness in life and her marriage depicts a scene of reality, where one draws a number of lessons from what she undergoes. I total agree with angel analysis of the story, the main character analysis is on point and very informing. Joe undergoes a series of related issues and he is left with no choice but to employ his detective skills; and all these he has to do in order to save lives. Jim analysis is also very informing and his contribution to the story’s plot is noted. Jim being younger and leaving his girlfriend enlightens him and when he teams up with Joe we find him being his side